Amy conducted an on-board nutrient addition culturing experiment in a research cruise trip to the Southern Ocean. In every station, she collected seawater and conducted incubation experiment using the seawater as the base medium. In one of the stations, she found that only when she added both Fe and Mn into seawater, there was an increase of chlorophyll a concentration detected after 24 hours of incubation. Which of the following statement is correct?1. Mn is the primary limiting factor in this area2. Fe is the primary limiting factor in this area3. Amy found Fe and Mn co-limitation in this area4. None of these answers is correct

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