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You are still sick to your stomach over just having made a 1 ug/uL primer stock under pressure from your lab mates.And sure enough, it is time to pay the price for the lab's lazy attitude towards making primer stock solutions......Your PCR protocol specifies that you need to have a 10 uM working solution. So, you will need to dilute the primer stock you just made to 10 uM. A final volume of 100 uL will enable you to do all of the necessary experiments. The primer is a 26-mer. Assume that the average molecular weight of a nucleotide is 330 g/mol.What is the correct volume of primer stock and water you need to combine to make this dilution? The volumes in the answers below are listed in a way that is compatible with the pipettes we use in class (i.e. rounded).
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