Why is SDS-page carried out in a pH buffered solution?Choice A, Using a pH buffered solution disrupts protein-protein interactions that occur under physiological conditions, which are not pH buffered, allowing for complete separation across the gel.(Choice B, Using a pH buffered solution ensures that SDS-bound polypeptides maintain the desired electrical charge.(Choice C, Using a pH buffered solution maintains the native conformations of the proteins being separated.(Choice D, Using a pH buffered solution prevents the autoionization of water, inhibiting the production of reactive hydronium and hydroxide species.

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