Students investigated the effect of light on the carbon cycle in aquatic ecosystems by performing the controlled experiment summarized below. The students placed equal amounts of water (pH 7.0) from a large aquarium in glass beakers. The students transferred aquatic plants from the aquarium to several of the beakers, and then they placed equal numbers of the beakers in the light or the dark (Figure 1: groups I and II). Similarly, the students transferred goldfish from the same aquarium to other beakers, and then they placed equal numbers of those beakers in the light or dark (Figure 1: groups III and IV). Finally, the students placed an equal number of beakers containing water only in the light or dark (Figure 1: groups V and VI).After exposing the samples to light or dark for one hour, the students recorded the pH of the water in each beaker. Carbon dioxide dissolved in water will lower the pH of an aqueous solution. In the experiment, the students used changes in pH to monitor changes in the amount of carbon dioxide in the water. For each treatment group, the students calculated the mean pH and standard error, as documented in the table below.The results for treatment groups V and VI could suggest which of the following questions about the design of the experiment?ADo the glass beakers prevent light from reaching the test samples?BIs the method used for measuring pH harmful to aquatic organisms?CIs the availability of carbon dioxide a limiting factor in aquatic ecosystems?DDoes the aquarium water contain living microorganisms?

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