Specimen: piercing and extracting fluid from ulcus durum, Blood and CSF (serological, test for AB in patients serum)Transportation: BACTECCultivation: do not grow!Direct:o Dark field microscopy - spirochetes are regularly curved and motileo Florescence microscopyo PCRIndirect:Serological test (nontreponemal):- Veneral disease reserach laboratory test (VDRL) (only for CSF)- Rapid plasma reagin test (RPR) (only for blood plasma)- Trust testSerodiagnostics (treponemal):- Specific AB detection (IGM and IgG)- Non specofic AB detection (cardioloipin)!st stage: PCR, microscopy2nd and 3rd stage:- Fluorecent treponemal antibody absorption (FTA-ABS)->prove of antibodies specifically against Treponema pallidum and not any other Treponema bacteria.(CSF)- Treponema pallidum particle agglutination (TP-PA test)->2weeks after infection- Treponemal hemagglutination (TPHA)- Microhemoagglutination T.pallidum (MHA-TP)

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