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Silmukointi vaiheittain:1. (1) kiinnittyy eksonin ja intronin rajalle 5'-päähän. U2AF ja BBP tunnistavat branch-point-site:n.2. (2) korvaa BBP:n ja U2AF:n. 3. Paikalle saapuvat U4, U5 ja U6 snRNA:t. U4:n ja U6:n välisten sidosten katkeamisen seurauksena (3). Introni muodostaa lasson. 4. Introni irroitetaan ja eksonit liitetään yhteen. Eksonien liitoskohtaan lisätään (4), joka on merkki onnistuneesta silmukoinnista.
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