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- Ring of bark removed from woody stem causes a bulge around ring which has a higher concentration of sugars than below ring, showing downward flow of sugars - Radioactive tracing to track movement -Use of aphids to measure pressure, pierce phloem causing sap to flow out - sap flows out quicker near leaves than stem evidence of pressure gradient - If metabolic inhibitors are put in phloem translocation stops which is evidence of active transport

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