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- Prendre les feuilles de tabac infectées, faire un liquide et le faire passer par le filtre. Normalement, l'agent pathogène devait rester dans le filtre.- Pourtant, quand il prend l'eau dans le filtre et injecte à une feuille saine, la feuille reste saine. L'agent pathogène n'est pas présent dans la cellule.- Injecter la feuille saine par l'eau dans le filtrat, la feuille devient malade. L'agent pathogène reste dans le filtrat.

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