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På ett mikroskopsnitt utför man en kemisk reaktion av något slag för att påvisa exestens och lokalisation av en bestmäd substans.Man får en 'semi-kvantatit' uppfattning om var substansen finns. Kräver att snittet är relativt intakt.Ex: lipidfärgning för att skilja åt ipider eller van Geison för att identifiera kollagen.

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