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On Thu last week, Sarah obtained a volume of 1.7 mL crude lysate. The proteins in this lysate need to be mixed with the resin (a suspension of agarose beads with Ni2+ ions attached to them). The resin has been washed in 4 mL Rinse Buffer and then settled to the bottom of the tube, with the Rinse Buffer sitting on top of it. Which of the following answers describes the correct procedure for mixing the lysate with the chromatography resin?

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