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- Large, spore-forming (subterminal) Gr+ rods- Obligate anaerobes- These bacteria are subdivided into four groups based on phenotypic and genetic properties and certainly represent four separate species, although they have been historically classified within a single species, C. botulinum- Seven antigenically distinct botulinum toxins (A to G) have been described; human disease is associated with types A, B, E, and F

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