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La vernalisation nécessite un certain délai minimum pour être complète.Il n'y a pas de floraison si la période froide est trop courte..Les plantes annuelles hivernantes répondent tôt dans leur cycle de vie.Les températures optimales se situent autour de 1 à 7°C.La vernalisation rend le méristème apical compétent à fleurir après le froid.

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