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Först tradiotionell fixering sedan läggs vävnaden i en lösning för att göra om hårdvävnaden till mjukvävnad.Förderlar - Vävnaden är relativt välbevaradNackdelar - Urkalkningen kan ta lång tid. Vissa vävnader som tandemalj försvinner (de har inte en olöslig fibrös matrix)

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