An organism grown in nutrient rich broth to high turbidity always produces a bluepigment. When a large inoculum is transferred to a nutrient rich agar plate, it alsoappears blue. A researcher noticed that this never happens when very small coloniesare grown on low nutrient agar plates, however. What is the most plausible conclusion?1. A) Large populations enabled the differentiation of a subpopulation of cells thatcreated the blue pigment.2. B) Only high nutrient conditions provide enough energy for cells to produce thissecondary metabolite that appears blue.3. C) Production of the blue pigment production is linked to quorum sensing.4. D) The strong gradient from very high to low nutrient bioavailability inducesproduction of the blue metabolite

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