All of the following statements describe protein fractionation, EXCEPT?a) Ion-exchange chromatography relies on proteins with a net charge binding to a solid resin with the opposite chargeb) In affinity chromatography, ligands are used to purify the proteins that bind them.c) Gel-filtration chromatography depends on size, shape and net charge of the protein.d) If ion-exchange chormatography is used after salting out, dialysis must be performed in between the two methods.e) In affinity chromatography, the binding of a protein to a ligand is by specific non-covalent interactions.

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