*10.35 The enzyme Tsp45I recognizes the 5-bp site 5' -G-T-(either C or G)-A-C-3' . This site appears in exon 4 of the human gene for -synuclein, where, in arare form of Parkinson disease, it is altered by a single G-to-A mutation. (Note: Not all forms of Parkinson disease are caused by genetic mutations.)a. Suppose you have primers that can be used in PCR to amplify a 200-bp segment of exon 4 containing the Tsp45I site, and that the Tsp45I site is 80 bp from the right primer. Describe the steps you would take to determineif a patient with Parkinson disease has this alpha-synuclein mutation.b. What different results would you see in homozygotes for the normal allele, homozygotes for the mutant allele, and in heterozygotes?c. How would you determine, in heterozygotes, if the mutant allele is transcribed in a particular tissue?

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