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La limite de séparation de l'œil est de 100 µm. Cette limite de séparation se définit par la distance minimale entre deux points qui permet de les distinguer d'un de l'autre. Pour la calculer, nous devons faire : . Celle-ci dépend de plusieurs paramètres, tel que la densité des photorécepteurs (cônes) au niveau de la zone la plus sensible de la rétine. Les aigles ont une densité en photorécepteurs plus importante que nous.

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