Bioinformatics Questions

If you were sequencing the genome of an organism and don't know any of the sequence, which method should you use?a. Maxam-Gilbert sequencingb. shotgun sequencingc. primer walkingd. none of these

What is a clade?a. a cluster of branches that group together from multiple points b. how far apart 2 branches arec. a cluster of branches that group together from a single point d. none of these

Why I'd bootstrapping commonly used to test the accuracy of a tree?a. it can determine the robustness of the tree placement b. it can test consistency of a clade placementc. it can test how efficiently a tree was made d. all of these

When choosing a command line bioformatics which of the following should be a consideration?a. the applicationb. further analysis to be preformedc. source of dataD. all of these are considerations

Which of the following is not a reason to identify motifs?a. to characterize an unknown proteinb. to determine the function of a proteinc. the identify related proteinsd. all of these

Why is it essential to have highest quality DNA for massively parallel sequencing?a. low quality DNA leads to low quality resultsb. inhibitors can inhibit the sequencing reactionc. the cost of the reaction is much higher than Sanger sequencing D. all of these

What is the basis for the MinION sequencer?a. nanoporesb. sequencing by litigationc. sequencing by synthesis d. none of these

Why is Maxam-Gilbert sequencing not used today? a. it is technically challengingb. it can be expensivec. other techniques were developedd. all of these

If your were to align sequences using a web based program, which would be the best option?a. ClustalOmegab. ClustalWc. MEGAd. TreeFinder

What types of user-interface based bioinformatics programs are there?a. propriety freeware b. government funded software that is freec. propriety fee-based programsd. all of these

How are didoxynucleotides labeled?a. radioactive phosphateb. fluorophores c. both of thesed. neither of these

Why would a researcher add a fluorescent tag to a protein through genetic engineering?a. to quantify the concentrationb. to localize the protein in a cellc. to make it shiny d. none of these

What is the major difference between qPCR and digital PCR?a. digital PCR is not quantitative b. digital PCR happens in silicoc. digital PCR separates the sample into thousands of subsamples for quantization making it more sensitive d. there is no difference

What is an infectious clone?a. a transformed bacteriab. a cDNA copy of a viral genomec. a bacteria that has multiple antibiotic resistance genes D. a plasmid that kills bacteria

Which of the following does not involve mathematics/statistics?a. structure learningb. experimental designc. preprocessing of datad. all of these are involved

Why are vector constructs verified before use?a. if the construct is wrong, then the downstream experiments won't workb. YOLOc. verification is an uneeded step that adds expense to a projectD. none

What type of chemistry does Illumina sequencing use?a. nanoporesb. pyrosequrncing c. reversible terminator dyes d. none of these

What are the benefits of using affinity Binding for protein purification?a. can get a pure extractb. specific for the tag on the proteinc. simplifies the extractionD. all of these

What is the origin for reverse transcriptase?a. algaeb. retro- and parareteoviruses c. bacteriad. it was made entirely in a lab with no natural origins

What is a motif?a. a type of proteinb. a conserved sequence of amino acids that has a functionc. something the professor made upd. none